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中国癌症防治杂志

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (1): 29-33.doi: 10.3969/j.issn.1674-5671.2012.01.07

• 基础研究 • 上一篇    下一篇

雌激素诱导子宫内膜癌细胞血管因子生成的作用

  

  1. 广西医科大学附属肿瘤医院妇瘤科；广西医科大学研究生学院；贵港市人民医院妇产科
  • 出版日期:2012-03-25 发布日期:2012-04-16
  • 通讯作者: 张洁清 E-mail:zjg201008@hotmail.com
  • 基金资助:

    国家自然科学基金（81160318）；广西自然科学基金资助项目（桂科回0991016）

Effect of estrogen on growth of endometrial cancer cells and expression of angiogenic factors

  • Online:2012-03-25 Published:2012-04-16

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被引次数

13

摘要： 目的 探讨雌激素诱导子宫内膜癌细胞HEC-1A血管因子生成的机制，阐述雌激素在子宫内膜癌发生中的作用。方法 以雌激素（E2组）作用于HEC-1A细胞30min，雌激素受体抑制剂（ER组）、AKT抑制剂（AKT组）、NF-κB抑制剂（NF-κB组）分别预处理HEC-1A细胞1h，各加入雌激素作用30min后；采用qRT-PCR检测各组细胞内血管因子mRNA表达，Western blot检测血管因子蛋白的表达情况。结果 E2组的血管因子mRNA及蛋白的表达均明显高于对照组（P<0.05）；ER组、AKT组及NF-κB组的血管因子mRNA和蛋白的表达均明显低于E2组（P<0.05）。结论 雌激素在子宫内膜癌HEC-1A细胞中起到信号传导的作用，可调节血管生成因子的表达，参与子宫内膜癌发生、发展的过程。

关键词: 雌激素, 子宫内膜癌, 血管生成因子

Abstract: Objective To explore the role of estrogen in endometrial cancer by examining the expression of angiogenic factors in the estrogen-induced endometrial cell line HEC-1A. Method Real-time quantitative PCR and Western blots were used to analyze the mRNA and protein expression of angiogenic factors in HEC-1A cells after stimulation with estrogen for 30 min with or without 60-min pretreatment with inhibitors against the estrogen receptor，the AKT kinase pathway，and the NF-κB pathway. Result Real-time PCR and Western blots showed that mRNA and protein expression of angiogenic factors were significantly higher in the estrogen-stimulated group than in the unstimulated control group（P<0.05）. Pretreatment with signaling pathway inhibitors significantly reduced mRNA and protein expression of angiogenic factors below the levels in the estrogen-stimulated group that was not pretreated （P<0.05）. Conclusion Estrogen induces the production of angiogenic factors by activating signal transduction pathways.

Key words: Estrogen, Endometrial cancer, Angiogenic factors